FastQCFastQC Report
Thu 2 Jun 2016
DRR028245_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028245_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences941222
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATACC4000.065.240036145
GGTATCA9250.050.1518331
TCAACGC14400.048.826754
GTATCAA15650.048.6321561
ATCAACG14850.047.8352663
CGACGAA2450.047.339706145
CAACGCA16350.043.003385
AACGCAG17200.041.7210586
TATCAAC19450.036.8947142
ACGCAGA19750.036.3342937
GTACATG29150.036.0560381
CGCAGAG20300.035.349868
CATACGC900.003631783932.2155883
TACATGG30750.031.8227142
TACCGTC1204.0627655E-430.2021127
ACATGGG31900.029.5393713
GCAGAGT24900.027.9460539
TACACCG2954.838512E-1027.0283345
GTACCGT1450.001227862824.9948526
CATGGGG24550.023.9156464
Produced by FastQC (version 0.11.5)