FastQCFastQC Report
Thu 2 Jun 2016
DRR028245_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028245_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences941222
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10320.109644695937834No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9520.10114510710544378No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14050.069.2767261
GTATCAA24850.049.106811
TCAACGC26700.044.2592284
ATCAACG26850.044.011973
CAACGCA27700.042.6614235
TATAACG1201.9736581E-742.290642
AACGCAG29100.040.6089826
GTACATG32300.040.2540321
TATCAAC31350.037.694462
TACATGG34100.036.3554272
ACATGGG34800.034.5824973
ACGCAGA34700.034.0553747
CGCAGAG34650.033.8952838
TAACGCA1652.3866814E-630.7568324
CATGGGG26100.029.1659624
GCAGAGT41100.028.7523469
CAGAGTA39150.026.110489
GAGTACA24800.025.7744791
ATAACGC2303.142357E-522.0646843
GACCGGC1650.002601111821.9691667
Produced by FastQC (version 0.11.5)