FastQCFastQC Report
Thu 2 Jun 2016
DRR028244_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028244_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1323191
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA18450.042.4274561
GTACATG47550.039.631671
TCAACGC18850.038.0665444
ATCAACG19450.036.8922543
TACGCGC800.002039579736.240126
GGTATCA15700.035.547631
TACATGG50750.035.4189152
ACATGGG52050.033.5595323
CAACGCA23500.031.151085
GAGTACA35350.030.7554051
TATCAAC24850.030.042112
AGTACAT36500.028.9920942
CGTATAT1306.49441E-427.8770143
AACGCAG26450.027.6767646
CATGGGG40850.027.3242514
ACGCAGA28150.025.2329087
CGCAGAG28100.025.0198678
CAGAGTA32750.021.4674289
TGACGCG1700.003098212621.317717145
GCAGAGT35250.020.1505348
Produced by FastQC (version 0.11.5)