FastQCFastQC Report
Thu 2 Jun 2016
DRR028244_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028244_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1323191
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14640.11064162316702576No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13420.10142148790310695No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21900.069.314771
GTATCAA34450.057.7676961
TCAACGC39000.049.8183064
ATCAACG39700.049.122513
CAACGCA41300.047.0439225
AACGCAG44250.044.0714956
TATCAAC46000.042.5524642
ACGCAGA49650.039.1322027
CGCAGAG50400.038.4060338
GTACATG50600.034.593161
TACATGG52700.031.6399652
GCAGAGT62150.030.9117459
GTGGTAT12000.030.8682921
ACATGGG54750.030.1904433
GAGTACA37900.025.2963811
TACACCG3501.2914825E-1024.8560435
GTATAGG5900.024.6207731
TTTCGCG1902.1943473E-422.8937239
CATGGGG41300.022.8198134
ACACCGT3503.5051926E-922.7847066
Produced by FastQC (version 0.11.5)