FastQCFastQC Report
Thu 2 Jun 2016
DRR028243_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028243_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1258423
Sequences flagged as poor quality0
Sequence length151
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA20550.046.9098131
TCAACGC20800.045.3005944
GTACATG47400.044.4977951
ATCAACG21600.043.287233
TACATGG48900.040.4648252
GGTATCA15550.040.085931
CAACGCA24800.037.7017825
AACGCAG26450.035.3498766
ACATGGG55100.035.122353
TATCAAC27000.034.3613362
ACGCAGA28550.032.749717
CGCAGAG29350.031.116188
CATGGGG42100.029.0956764
GAGTACA35250.025.9080831
GCAGAGT35450.024.535169
GAGCGAC5300.023.248604145
ATGGGGG26750.023.031335
AGTACAT36400.022.5009542
GTATTAG8950.021.865761
AGAGTAC30300.020.8113615
Produced by FastQC (version 0.11.5)