FastQCFastQC Report
Thu 2 Jun 2016
DRR028243_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028243_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1258423
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12700.10091996093523402No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20950.060.343931
GTATCAA33150.059.614751
TCAACGC39250.049.500934
ATCAACG39500.049.1876343
CAACGCA41150.047.2153475
AACGCAG43850.044.3081326
TATCAAC45100.043.4015732
ACGCAGA51050.037.632967
TAGACCG800.002037269536.2483525
CGCAGAG53050.036.077538
GTACATG49850.035.708311
TACATGG49800.034.3558652
ACATGGG51500.032.2363973
GCAGAGT59700.032.058849
GTATAGG7000.026.9863191
TTTCGCG1907.159888E-626.7093099
GTGGTAT12550.026.6306931
CATGGGG41100.025.9294784
GAGTACA38500.024.3443051
TATAGGG6650.023.9838732
Produced by FastQC (version 0.11.5)