FastQCFastQC Report
Thu 2 Jun 2016
DRR028242_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028242_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1190330
Sequences flagged as poor quality0
Sequence length151
%GC45

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGCGT450.00964754248.323249
GACGACC6450.048.323235145
GGTATCA12700.046.8012471
GTATCAA18600.045.595311
TCAACGC19250.041.4199184
ATCAACG19900.040.431253
GTACATG43800.037.4008641
TACATGG45350.035.323382
TATCAAC24650.034.6986352
CAACGCA23400.034.0740785
AACGCAG24550.032.477946
ACATGGG47900.031.3243563
GAGTACA31950.030.8542731
ACGCAGA25600.030.2964067
GTATACG1804.708596E-628.1885551
GTATAGG5750.027.7333371
CGCAGAG28250.027.4544438
AGTACAT34000.027.0752282
CATGGGG36500.024.2278164
CCGACGC4353.6379788E-1223.328459145
Produced by FastQC (version 0.11.5)