FastQCFastQC Report
Thu 2 Jun 2016
DRR028242_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028242_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1190330
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13900.11677433988893837No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12820.10770122571051724No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19700.066.014681
GTATCAA31750.057.893591
TCAACGC35750.050.697464
ATCAACG37050.049.114283
CAACGCA38750.046.5854075
AACGCAG40450.044.806786
TATCAAC42900.042.2478832
ACGCAGA45650.039.0675857
CGCAGAG47600.037.0102128
GTACATG42700.035.39071
TACATGG44050.033.4096872
GCAGAGT54550.031.4974829
ACATGGG45700.030.9343283
GTGGTAT10800.030.2720951
GCGTAAT1000.006012654429.0612091
GTATAGA8250.027.2999211
CATGGGG34000.026.2269924
GTCTAAC2258.9439345E-725.8321841
TGGTATC12200.024.3638732
GAGTACA33050.023.3017251
Produced by FastQC (version 0.11.5)