FastQCFastQC Report
Thu 2 Jun 2016
DRR028241_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028241_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences923258
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA15150.050.714521
TCAACGC14600.048.6532944
ATCAACG15750.045.1008343
GGTATCA10350.044.82071
CAACGCA17300.041.0600135
TAGATCG907.49205E-540.26865
AACGCAG18100.039.645666
TATCAAC19550.037.075952
GTACATG29100.036.3662831
ACGCAGA20050.035.789857
TACATGG29850.033.2671242
CGCAGAG21850.032.5097548
ACATGGG32500.028.7703693
GTATACG1555.5085162E-528.0581231
GTACGGT1050.007716723727.6127576
TACCTGG11700.027.2587472
GCAGAGT26400.027.1813059
GTACCTG12900.025.8468251
CATGGGG24500.024.2597774
GAGTACA22600.023.7335281
Produced by FastQC (version 0.11.5)