FastQCFastQC Report
Thu 2 Jun 2016
DRR028241_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028241_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences923258
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12810.13874778231003684No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12450.13484854720998898No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14250.063.1855621
GTATCAA26050.056.5847471
TCAACGC27150.053.672854
ATCAACG27300.053.3779453
CAACGCA29000.050.248895
AACGCAG31150.047.4788866
TATCAAC31400.046.63912
ACGCAGA35700.041.0215077
CGCAGAG37500.039.052478
TAACGCA1604.589856E-836.24924
GCAGAGT41550.035.0714269
TATAACG1251.2651737E-534.7992322
GTACATG28100.032.04251
TACATGG28600.030.6724012
ACATGGG29450.029.294773
TAGATCG1050.00770897827.6184395
CTGTCGC4350.026.666089
TATCTCG1409.995293E-425.8922885
TACCTGG11300.025.021572
TCGTGCG1450.001226529224.999459
Produced by FastQC (version 0.11.5)