FastQCFastQC Report
Thu 2 Jun 2016
DRR028240_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028240_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences754023
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA12600.048.3179241
ATCAACG13250.042.665643
GGTATCA8750.042.2436751
TCAACGC13550.041.7210164
GTACATG28250.036.687421
CAACGCA15600.036.2384455
TATAACG800.002039486536.2384452
GCGTATA800.002039486536.2384451
GTACGGA1406.6232496E-736.2384456
TACATGG28600.034.4645352
AACGCAG16850.034.410396
TAGTACG2750.034.2618034
AGTACGG1501.1362281E-633.8225485
TATCAAC17550.032.6249242
ACGGATG1851.6473132E-731.341368
ACGCAGA18600.031.1728537
ACATGGG31150.029.3164963
CGCAGAG19550.029.2873368
GTATATA5650.025.6555371
GAGTACA21150.025.0156631
Produced by FastQC (version 0.11.5)