FastQCFastQC Report
Thu 2 Jun 2016
DRR028240_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028240_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences754023
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13870.18394664353739873No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13110.1738673753983632No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13050.071.195181
GTATCAA23800.064.961171
TCAACGC27500.055.362934
ATCAACG28200.054.2457583
CAACGCA29550.051.7675325
AACGCAG30900.049.7404636
TATCAAC31550.049.1752852
ACGCAGA35750.042.3840757
CGCAGAG36700.041.0893948
GTGGTAT6500.039.0846141
GCAGAGT42800.034.725029
GTACATG28150.032.489521
TACATGG28250.031.3093342
ACATGGG29000.029.7496173
TGGTATC9050.026.4361252
CTAGACG1100.0096707126.3632984
CATGGGG20650.024.224874
TACGGGT1550.001808212723.3867974
TACACTG7750.023.3867975
TCGATTG1600.002174819422.655965
Produced by FastQC (version 0.11.5)