FastQCFastQC Report
Thu 2 Jun 2016
DRR028239_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028239_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences964731
Sequences flagged as poor quality0
Sequence length151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA11250.047.683131
GTATCAA15450.042.697111
CGACGAC2700.040.272907145
ATCAACG15250.039.92963
TCAACGC15550.039.1592564
GTACATG33750.035.2253721
CAACGCA17850.034.1135255
TACATGG34750.032.9600452
AACGCAG19100.031.8809646
TATCAAC19650.031.7264462
ACATGGG36300.030.554163
ACGCAGA21450.028.7261337
GAGTACA24800.027.7688221
CGCAGAG22500.027.385588
TAGATCG1100.00967711526.360455
ATACGGT1400.001000138125.889736
ATCTACC8550.025.435522145
AGTACAT26150.024.3947582
GCGAGTA1500.001495286624.1637489
CATGGGG28300.024.0783634
Produced by FastQC (version 0.11.5)