FastQCFastQC Report
Thu 2 Jun 2016
DRR028239_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028239_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences964731
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12750.059.2548071
GTATCAA21100.048.888461
TCAACGC22450.045.2098854
ATCAACG22600.044.909823
CAACGCA24200.041.9405755
AACGCAG26350.038.793616
TATCAAC26850.038.3412062
TCTAGCG800.002036954536.248642
GTACATG32400.034.528381
ACGCAGA30400.033.863867
CGCAGAG31650.032.2973678
TACATGG32300.031.6474172
ACATGGG32450.030.1606563
GCAGAGT36650.027.0999379
TATAACG1100.00967318826.3626462
GAGTACA25100.025.7582741
CGTAGAC2859.89894E-925.4376413
CATGGGG24200.024.2656174
CAGAGTA34950.023.6471829
GTGGTAT8100.023.3178671
Produced by FastQC (version 0.11.5)