FastQCFastQC Report
Thu 2 Jun 2016
DRR028237_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028237_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1171016
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATACC4650.054.557953145
GGTATCA12500.054.5080681
GTATCAA19750.051.0141531
TCAACGC19900.049.9011424
ATCAACG20150.049.2820173
CAACGCA23950.041.7653925
AACGCAG25500.038.942466
TATCAAC27250.037.771552
ACGCAGA27600.035.716827
CGCAGAG27750.035.5237588
GTACATG44700.033.242171
TACATGG44250.031.9421652
ACATGGG45100.029.572243
CGACGAA3301.8189894E-1228.554358145
GCAGAGT35650.026.2284839
GAGTACA32900.026.2176671
CATGGGG32900.025.5567174
CGTAATA1801.5227606E-424.1613792
CGTATAC1600.002178138822.6512933
AGTACAT37250.021.9884782
Produced by FastQC (version 0.11.5)