FastQCFastQC Report
Thu 2 Jun 2016
DRR028237_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028237_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1171016
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13900.1187003422668862No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13810.11793177889969052No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18750.074.013041
GTATCAA30950.055.1675031
ATCAACG34600.048.819193
TCAACGC35000.048.054134
CAACGCA37500.044.850525
AACGCAG39550.042.525786
TATCAAC40950.041.4259722
ACGCAGA43750.037.9461947
CGCAGAG45350.036.6074038
GTACATG40150.034.925921
TACATGG41200.032.376612
GCAGAGT52750.031.4719629
GTGGTAT9950.031.399411
ACATGGG44400.029.2267723
GAGTACA30500.026.680521
CATGGGG34500.025.2158094
TATACTG8500.024.7337425
AGTACAT32650.024.4241962
TGGTATC12600.023.0144312
CAGAGTA50000.022.0386189
Produced by FastQC (version 0.11.5)