FastQCFastQC Report
Thu 2 Jun 2016
DRR028236_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028236_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1778840
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC19200.045.6832584
ATCAACG20100.043.6377373
CAACGCA23000.038.450765
GGTATCA17750.036.7550541
GTATCAA25450.035.888531
GTACATG46300.035.6965941
AACGCAG25450.034.7492146
TGACGCG1551.4675752E-632.73703145
TCTCGGG18700.031.78668145
CGCAGAG27750.031.0854328
ACGCAGA28250.030.7918477
ACATGGG45400.030.4965323
TACATGG48050.029.7197912
TATCAAC31350.028.6719382
TAGACCG1050.00771568127.6149085
TATACCG2156.137125E-726.9727025
TAGATCG3052.0401785E-823.766935
GTATAGG10950.021.846041
CATGGGG32200.021.836794
CATGGGA19550.021.5057284
Produced by FastQC (version 0.11.5)