FastQCFastQC Report
Thu 2 Jun 2016
DRR028236_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028236_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1778840
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC24600.053.9308244
ATCAACG26600.050.1484223
CAACGCA26500.050.0640875
GTATCAA30200.046.649711
AACGCAG30650.043.2854276
GGTATCA19800.040.344251
CGCAGAG34350.038.200848
ACGCAGA35200.037.2783787
TATCAAC38900.035.0371932
GTACATG49650.034.8106271
TACATGG51500.030.8288962
ACATGGG50350.029.8051933
GCAGAGT46500.027.59579
ATTACGG2705.5715645E-926.8508058
GAGTACA39350.024.7294351
CATGGGG32400.024.1657264
GTATAGG11650.023.0637531
AGTACAT43450.021.6907562
GACCGCG1850.00504953119.5938327
CTCCGAC4102.1642336E-819.455381145
Produced by FastQC (version 0.11.5)