FastQCFastQC Report
Thu 2 Jun 2016
DRR028235_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028235_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2032781
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAC6300.048.320988145
TCAACGC21300.047.98074
ATCAACG22250.045.9320833
GTATCAA26700.041.8057981
CAACGCA25850.039.535355
GGTATCA18250.038.1272431
AACGCAG28400.036.2407386
CGCAGAG30500.033.507838
ACGCAGA30850.033.3626257
GTACATG57100.031.3536341
TCTCGGG17250.031.093504145
TATCAAC36300.029.5516762
TACATGG61200.026.7660352
ACATGGG59200.026.4459463
GAGTACA45050.024.9381331
GCAGAGT43300.023.435129
CATGGGG45050.022.6856564
TATATCG2704.458456E-621.4759925
AGTACAT49800.020.95852
GTATTAG14100.020.562121
Produced by FastQC (version 0.11.5)