FastQCFastQC Report
Thu 2 Jun 2016
DRR028235_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028235_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2032781
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC28850.044.4785844
ATCAACG30600.042.171793
GGTATCA24300.042.1297071
CAACGCA32500.039.7063675
GTATCAA33800.039.3106271
AACGCAG34450.037.248396
GTACATG59550.034.017161
CGCAGAG38600.032.6802568
ACGCAGA39950.031.9388587
TATCAAC42700.031.2401732
TACATGG59300.030.074882
ACATGGG57900.029.6751733
GAGTACA44100.025.6839391
CATGGGG42000.024.165864
GCAGAGT53550.023.6920179
GTATTAG15450.022.0874211
GTATAAT14050.021.7044411
TGTACCG2404.366858E-521.1451245
AGTACAT48300.021.013792
AGGACCG2959.572959E-619.6603585
Produced by FastQC (version 0.11.5)