FastQCFastQC Report
Thu 2 Jun 2016
DRR028234_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028234_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1788163
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGACC12850.055.284393145
TCAACGC21150.041.4719664
GGTATCA19600.040.6833081
CCGACGC5200.040.427254145
ATCAACG22700.038.640183
GTATCAA25850.036.175021
CAACGCA25150.034.5877955
GTACATG55950.033.815831
ACATGGG54100.031.6223663
AACGCAG28700.030.5620966
TACATGG58000.030.3709162
TCTCGGG17550.028.500443145
TGACGCG1050.007715109727.615334145
GAGTACA42450.027.493361
ACGCAGA32850.026.4804577
TATCAAC34700.025.6953932
CGCAGAG32200.025.6642512
CATGGGG39550.023.094244
GTATACG2303.1470736E-522.0622521
AGTACAT47700.021.883852
Produced by FastQC (version 0.11.5)