FastQCFastQC Report
Thu 2 Jun 2016
DRR028234_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028234_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1788163
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC24350.045.850534
CAACGCA26350.042.6455465
ATCAACG26250.042.5318223
GTATCAA29000.041.0777971
GGTATCA20600.040.197491
AACGCAG28700.038.901066
GTACATG53700.034.7632331
ACGCAGA35400.031.5384277
TTTCGCG1153.163748E-431.520629
ACATGGG52550.031.1787223
TACATGG55300.030.677032
CGCAGAG36000.030.4086448
TATCAAC39300.029.3310182
CTCCGAC5450.026.610514145
GAGTACA37750.025.976351
CATGGGG36300.025.3641154
GCAGAGT47700.023.1019049
GGGACCG1952.616489E-422.3069025
TATACTG17650.022.1805175
GGACCGC3208.4812564E-720.3899026
Produced by FastQC (version 0.11.5)