FastQCFastQC Report
Thu 2 Jun 2016
DRR028232_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028232_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1518107
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC18600.047.9292374
ATCAACG19950.044.685913
CAACGCA21800.040.5612835
GTATCAA23150.040.074431
TCTCGGG11050.040.010677145
AACGCAG23900.036.997326
GTACATG48200.036.840661
GGTATCA16100.035.1137431
CGAAACG850.002746447234.107464145
CGCAGAG26150.034.091168
TACATGG48700.033.4858972
ACGCAGA26450.033.4304737
ACATGGG46750.032.867193
TGACGCG2008.983989E-932.615265145
TATCAAC29000.030.990752
GAGTACA34650.027.401631
CATGGGG35500.026.7455374
CTTAGGG12450.026.196999145
ACCGCGT1751.258693E-424.8497248
GCAGAGT35150.024.3312859
Produced by FastQC (version 0.11.5)