FastQCFastQC Report
Thu 2 Jun 2016
DRR028232_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028232_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1518107
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17350.054.4088751
TCAACGC26850.049.6802334
GTATCAA28200.049.182451
ATCAACG27200.049.0409623
CAACGCA29750.045.0811355
AACGCAG31900.041.8154956
TATCAAC36950.037.277712
ACGCAGA36850.035.608297
CGCAGAG36350.035.4997838
GTACATG47750.034.2164571
TACATGG46700.032.5996172
ACATGGG46050.031.1706333
CCGATCG1204.0605565E-430.2063929
GCAGAGT45950.027.767539
GTATAGG8500.027.3373851
CATGGGG32200.027.2420394
GTGGTAT11150.023.4451771
TTGTACG2502.2817876E-623.198519
GAGTACA33400.022.1758141
AGTACAT36450.020.2867622
Produced by FastQC (version 0.11.5)