FastQCFastQC Report
Thu 2 Jun 2016
DRR028231_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028231_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1572834
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGAC5250.059.365513145
TCAACGC21300.047.2999044
ATCAACG22250.045.2803573
GGTATCA15900.045.1297721
GTATCAA25000.041.1692921
CAACGCA24850.040.834455
GTACATG52200.037.2125431
TAGATCG2401.8189894E-1236.2405745
TCTCGGG13200.035.691475145
AACGCAG29350.035.067546
TACATGG52650.033.4528352
CGCAGAG30250.032.5865638
ACATGGG52700.032.3208163
ACGCAGA32450.031.0473947
TATCAAC32950.030.5762672
CATGGGG37700.024.4167254
ATCTACC15150.024.399593145
GTACGGA3352.1191227E-923.799786
AATCCGC3702.6193447E-1023.50746
TGACGCG1550.001811686823.381016145
Produced by FastQC (version 0.11.5)