FastQCFastQC Report
Thu 2 Jun 2016
DRR028231_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028231_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1572834
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC26600.050.4212044
ATCAACG27000.049.6742253
GGTATCA18200.049.485161
GTATCAA29350.048.503451
CAACGCA28100.047.7296835
AACGCAG31500.042.5779086
GTACATG51500.038.924811
TATCAAC38400.036.0599632
CGCAGAG37250.035.4216088
TACATGG51700.035.3372842
ACGCAGA37800.035.28987
ACATGGG50950.033.296213
CTAACGC1306.485909E-427.8836613
GCAGAGT47250.027.464679
GTATAAC7100.024.5514741
CATGGGG37300.024.2954164
TATATCG1952.616272E-422.3069295
GACCGGT1650.00260203321.9689457
CATGGGA20900.020.8126854
CTCCGAC3851.0501026E-820.71819145
Produced by FastQC (version 0.11.5)