FastQCFastQC Report
Thu 2 Jun 2016
DRR028230_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028230_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1812179
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACGCG1453.8562575E-1044.98999145
TCAACGC21750.040.3243644
ATCAACG22750.038.8704723
GGTATCA19600.038.0910151
CAACGCA24450.035.8713655
GTATCAA26800.035.430551
GTACATG52700.030.5340041
AACGCAG29000.030.4932146
CGCAGAG30050.028.7040988
ACGCAGA31600.027.75497
ACATGGG51900.026.8148423
TACATGG52950.026.6937772
GAGTACA38600.025.7261431
TCTCGGG22950.024.950876145
TATCAAC36800.024.4239122
GTATAGG12750.023.8770431
GTACAGG15500.022.9142571
GTACTAA8850.021.2946951
TGTACCG2404.372887E-521.141135
AGTACAT44800.020.8714732
Produced by FastQC (version 0.11.5)