FastQCFastQC Report
Thu 2 Jun 2016
DRR028230_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028230_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1812179
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19750.048.9091571
TCAACGC27650.045.0982134
GTATCAA30750.043.222671
ATCAACG29400.042.9069823
CAACGCA29000.042.748825
AACGCAG33200.037.7775736
CGCAGAG36750.033.1419458
TATCAAC40200.031.9207612
ACGCAGA38450.031.8651817
GTACATG52100.030.6683621
GTACGAC1453.492601E-529.9991723
CTCCGAC4400.028.014456145
ACATGGG51100.027.5237053
TATATCG2156.1306855E-726.9765
TACATGG52850.026.6123242
GAGTACA39000.025.6992281
GGACCGT2859.909854E-925.4378956
GCAGAGT48950.024.7337429
GACCGTG3102.4214387E-823.3864527
TAGATCG1550.001809343923.3864525
Produced by FastQC (version 0.11.5)