FastQCFastQC Report
Thu 2 Jun 2016
DRR028229_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028229_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1988459
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATACC10950.085.396805145
TCAACGC26450.050.6992034
ATCAACG27100.049.750653
GGTATCA19200.045.6813771
GTATCAA31600.045.6479261
CAACGCA33700.040.222295
CGACGAA5400.038.92873145
AACGCAG35650.038.022196
GTACATG67850.037.0710331
CGCAGAG35350.036.9095048
TATCAAC38250.036.5747152
ACGCAGA36650.036.193637
TACATGG69650.034.0315482
CCGCGTA900.003632482132.216079
ACATGGG72000.032.1153953
GAGTACA51400.027.2175661
GTACGGA3204.1836756E-1127.182316
GCAGAGT48700.026.4939129
CATGGGG49300.025.583354
CGCCGTT3501.3096724E-1024.853022145
Produced by FastQC (version 0.11.5)