FastQCFastQC Report
Thu 2 Jun 2016
DRR028229_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028229_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1988459
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21450.054.5125661
TCAACGC35200.052.313414
ATCAACG35300.052.165213
GTATCAA38600.049.6723371
CAACGCA38350.048.394565
AACGCAG42450.043.7204136
CGCAGAG45550.039.6308068
ACGCAGA47100.038.94237
TATCAAC48550.038.8245162
GTACATG66050.037.7154541
CCGATCG1406.615901E-736.248669
TACATGG68750.034.5878142
ACATGGG69050.033.4926073
GCAGAGT60150.029.167679
CATGGGG46850.028.3180584
TAGATCG3255.0931703E-1126.7682445
GAGTACA50900.025.2553541
CTCCGAC5200.023.705814145
GTATAGG10450.022.2398431
GTACAGG16400.021.6995261
Produced by FastQC (version 0.11.5)