FastQCFastQC Report
Thu 2 Jun 2016
DRR028228_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028228_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1727970
Sequences flagged as poor quality0
Sequence length151
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17650.048.4642031
TCAACGC24150.042.0237354
ATCAACG24750.040.7120863
GTATCAA27250.039.3712271
CAACGCA27350.036.841835
GTACATG56000.036.245471
AACGCAG28850.035.4288526
TACATGG57700.033.544342
TATCAAC32950.031.460412
ACATGGG61350.031.0759873
ACGCAGA33150.030.1772257
CATGGGG43150.027.8875924
CGCAGAG35900.027.6636738
GAGTACA40350.027.1279641
GTACGGA2051.2922228E-524.7530066
AGTACAT43600.022.9443822
AGTACGG3203.3936885E-822.653425
TTACACG4205.2750693E-1122.4376724
GCAGAGT42800.021.5101629
GTATTAG13000.021.189661
Produced by FastQC (version 0.11.5)