FastQCFastQC Report
Thu 2 Jun 2016
DRR028228_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028228_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1727970
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24790.1434631388276417No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23720.13727090169389514No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA28250.077.864561
GTATCAA47900.054.5610471
ATCAACG54300.047.664283
TCAACGC54750.047.4049384
CAACGCA57650.045.02035
AACGCAG60100.043.426296
TATCAAC63750.041.054832
ACGCAGA69200.037.610847
CGCAGAG70000.036.7667278
GTACATG53000.033.147841
GCAGAGT79550.031.8971989
TACATGG53800.031.668162
ACATGGG57150.028.7961393
TCGTATC2705.5606506E-926.855688145
GTACGAC1958.761401E-626.024843
GAGTACA42250.025.4302521
GTGGTAT15650.025.0492731
TTTCGCG1450.001227024524.9992319
CATGGGG42050.022.5855124
AGTACAT43250.022.4623872
Produced by FastQC (version 0.11.5)