FastQCFastQC Report
Thu 2 Jun 2016
DRR028227_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028227_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2171117
Sequences flagged as poor quality0
Sequence length151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18500.050.1526031
TCAACGC29250.044.8546944
ATCAACG29500.044.7202873
GTATCAA31000.044.6608431
CAACGCA35600.036.853935
GAGCGAC13350.035.292896145
AACGCAG37200.035.2688146
GTACATG70800.035.014511
TACATGG67600.034.634672
TATCAAC38700.034.0891082
ACATGGG71450.031.449573
ACGCAGA41050.031.25477
CGCAGAG42450.029.711658
TCTCGGG23000.028.364159145
GAGTACA51000.025.583361
CGTTATA1701.03317536E-425.5833572
CATGGGG55000.024.2499224
TGACGCG3001.7129423E-824.162062145
GCAGAGT52700.022.6949149
AGTACAT54800.021.4283982
Produced by FastQC (version 0.11.5)