FastQCFastQC Report
Thu 2 Jun 2016
DRR028227_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028227_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2171117
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA31750.067.689861
GTATCAA52600.054.6619451
TCAACGC58300.048.1246384
ATCAACG58800.047.8387073
CAACGCA62800.044.7916565
AACGCAG65450.043.1996276
TATCAAC67250.042.0433542
ACGCAGA75450.037.089687
CGCAGAG77450.035.8510938
GTACATG67850.034.7783621
TACATGG72250.031.4073682
GCAGAGT89750.030.5339099
ACATGGG69300.030.4428163
GAGTACA52050.025.387871
CTCCGAC8400.025.033627145
CATGGGG53400.024.8447654
GTGGTAT18300.023.8054181
AGTACAT56600.022.4154072
CAGAGTA85150.021.285339
TGGTATC20900.019.4252572
Produced by FastQC (version 0.11.5)