FastQCFastQC Report
Thu 2 Jun 2016
DRR028225_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028225_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2417270
Sequences flagged as poor quality0
Sequence length151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA36200.050.662181
GGTATCA23300.048.8445051
TCAACGC35950.048.796474
ATCAACG36350.048.4589273
CAACGCA42250.041.5203135
GACGAAA10900.039.90219145
GTACATG83650.039.4291531
TACATGG83650.038.4759182
AACGCAG46500.037.5695576
TATCAAC47850.037.1155662
ACGCAGA51150.033.303837
ACATGGG90650.033.2657623
CGCAGAG52800.032.1257938
CATGGGG68250.028.995594
GAGTACA59650.026.249151
GCCGACA7750.025.254225145
GCAGAGT64300.025.2527689
TGACGCG3601.8553692E-1024.16299145
AGTACAT62950.023.6064192
TATACCG3852.392335E-718.8283065
Produced by FastQC (version 0.11.5)