FastQCFastQC Report
Thu 2 Jun 2016
DRR028225_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028225_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2417270
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26290.10875905463601501No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26040.10772483007690495No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA36800.071.2233661
GTATCAA60350.056.4233251
TCAACGC67950.049.1854064
ATCAACG69050.048.506853
CAACGCA71500.046.844735
AACGCAG76050.044.1373836
TATCAAC77450.043.3404432
ACGCAGA89450.037.1201677
GTACATG80350.036.6862451
CGCAGAG91450.035.8327038
TACATGG81800.034.565682
ACATGGG85250.031.8905283
GCAGAGT102150.031.4405529
CTCCGAC9550.031.131792145
GTGGTAT18750.028.267321
GAGTACA60700.026.1950051
CATGGGG61900.025.7665864
TAGATCG3151.036824E-925.316695
AGTACAT63350.023.0029342
ACCGGTC2852.7829992E-722.8940418
Produced by FastQC (version 0.11.5)