FastQCFastQC Report
Thu 2 Jun 2016
DRR028224_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028224_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2029733
Sequences flagged as poor quality0
Sequence length151
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACGCG5350.052.837845145
GGTATCA20500.048.7932241
GTATCAA33000.045.6860541
ATCAACG31450.045.6329773
TCAACGC32200.044.5700954
GTACATG73250.039.0862241
CAACGCA37600.038.3618475
AACGCAG39300.036.3335576
TACATGG76000.036.145972
TATCAAC42350.034.7437632
ACATGGG82700.032.6917043
ACGCAGA44200.031.4856917
CGCAGAG46100.029.244648
TAGACCG1255.1629E-428.9930745
CATGGGG61350.028.1187924
GAGTACA52250.027.32841
CTTAGGG25500.027.003353145
GCAGAGT54750.023.0355939
AGTACAT55750.022.752412
TCTCGGG22200.022.201904145
Produced by FastQC (version 0.11.5)