FastQCFastQC Report
Thu 2 Jun 2016
DRR028224_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028224_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2029733
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26850.13228340870449462No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26000.12809566578461304No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA34250.071.416061
GTATCAA53450.054.3173871
TCAACGC62000.046.5387654
ATCAACG62550.046.0136453
CAACGCA65700.043.8075145
AACGCAG68250.042.2769746
TATCAAC71200.040.3216862
GTACATG79700.038.2487751
TACATGG81650.036.5817532
ACGCAGA80400.035.707767
ACATGGG85100.034.417183
CGCAGAG84200.033.837948
GTGGTAT16750.032.0659071
GCAGAGT95350.029.5768839
GAGTACA59000.026.941311
CATGGGG65200.026.908614
AGTACAT60350.025.3471222
TGGTATC20550.024.6950382
CAGAGTA91300.020.8837479
CATGGGA31300.020.6143264
Produced by FastQC (version 0.11.5)