FastQCFastQC Report
Thu 2 Jun 2016
DRR028223_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028223_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1831367
Sequences flagged as poor quality0
Sequence length151
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC24100.045.1158334
GTATCAA25250.045.0705681
GGTATCA18100.044.4528051
ATCAACG24800.043.84243
CGACGAC9650.042.815628145
CAACGCA26850.040.2250635
GTACATG59200.039.793891
TACATGG60150.036.996232
AACGCAG29700.036.6091426
ACATGGG64150.034.4633833
TATCAAC33150.033.0178032
ACGCAGA34700.031.1251567
ATCTACC16900.029.594917145
CGCAGAG37300.029.1499088
CATGGGG48950.028.7278924
GAGTACA42500.026.0949971
AGTACAT44500.023.6190682
GCAGAGT44450.023.1564169
AGAGTAC38450.022.2454095
TGACGCG3004.567628E-721.745832145
Produced by FastQC (version 0.11.5)