FastQCFastQC Report
Thu 2 Jun 2016
DRR028223_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028223_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1831367
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA26600.063.606161
GTATCAA41500.054.2423551
TCAACGC44200.049.8627324
ATCAACG44450.049.5822873
CAACGCA45950.047.9637155
AACGCAG49250.044.60276
TATCAAC54200.041.4655232
ACGCAGA57500.037.9510157
CGCAGAG58600.037.2386258
GTACATG60800.035.4713551
TACATGG62450.032.6211052
ACATGGG63150.030.8818783
GCAGAGT69450.030.690219
GAGTACA46700.025.811671
GTGGTAT15600.025.6013581
CATGGGG49100.025.3963744
CTCCGAC7200.024.17055145
TCGTATC3402.5029294E-923.45965145
AGTACAT49000.022.9329742
GGACCGT2959.571866E-619.6604166
Produced by FastQC (version 0.11.5)