FastQCFastQC Report
Thu 2 Jun 2016
DRR028221_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028221_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1712395
Sequences flagged as poor quality0
Sequence length151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATACC12800.056.064983145
GGTATCA18150.052.7185861
TCAACGC24300.043.5524944
GTATCAA26550.042.864881
CGACGAA6350.041.095592145
ATCAACG25900.040.5821153
GTACATG54100.037.1149561
CAACGCA28550.036.56145
CTAGCGT1251.2670416E-534.794274
AACGCAG30450.034.518126
TACATGG56550.034.0969472
TATCAAC35050.031.4356212
ACGCAGA33500.030.50997
ACATGGG61800.030.144713
TCTAGCG1453.4957608E-529.9950583
CGCAGAG34350.029.5438988
GAGTACA41000.028.2880231
CATGGGG47800.025.9319214
AGTACAT42200.025.250582
GACGATC5000.023.196178145
Produced by FastQC (version 0.11.5)