FastQCFastQC Report
Thu 2 Jun 2016
DRR028221_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028221_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1712395
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22500.13139491764458552No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21090.12316083613885816No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA27600.074.160081
GTATCAA43600.052.106031
TCAACGC48700.046.445994
ATCAACG50150.045.536773
CAACGCA52200.043.3317955
AACGCAG55350.040.996746
TATCAAC57150.039.707822
ACGCAGA65000.034.464167
CGCAGAG65850.034.0192958
GTACATG55300.032.4191741
TACATGG56600.030.1022822
GCAGAGT77450.028.268859
ACATGGG58250.028.1277563
CTCCGAC6050.027.56664145
GAGTACA43150.024.726731
GTGGTAT13000.024.5662841
AGTACAT44650.022.8952642
CAGAGTA74250.021.578369
CGCAGTA3951.4133548E-820.1903382
AGAGTAC69700.019.9705985
Produced by FastQC (version 0.11.5)