FastQCFastQC Report
Thu 2 Jun 2016
DRR028220_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028220_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1896595
Sequences flagged as poor quality0
Sequence length151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19600.049.9237251
TCAACGC25950.042.7349624
GTATCAA27850.042.682311
ATCAACG26450.042.2011533
CAACGCA29700.037.3391345
GTACATG61250.037.276381
TACATGG61800.036.0063552
AACGCAG32150.034.7191436
TGACGCG2653.6379788E-1232.821968145
TATCAAC35150.032.580752
ACATGGG70250.031.1594583
ACGCAGA37000.029.5804317
CATGGGG49950.028.0064
CGCAGAG39200.027.7354038
GAGTACA46700.026.5404621
TGTACCG3052.0425432E-823.7645425
AGTACAT49650.023.5036832
GCAGAGT45500.022.3021099
AGAGTAC39150.021.8463825
TCTCGGG19750.021.285809145
Produced by FastQC (version 0.11.5)