FastQCFastQC Report
Thu 2 Jun 2016
DRR028220_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028220_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1896595
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27080.14278219651533405No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26140.13782594597159648No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA27400.071.538191
GTATCAA49750.059.6835941
TCAACGC56250.052.3264164
ATCAACG57000.051.637913
CAACGCA59700.049.181095
AACGCAG64150.045.8824776
TATCAAC64750.045.6836472
ACGCAGA72550.040.370257
CGCAGAG73650.039.3735668
GTGGTAT14550.035.4258351
GCAGAGT85350.033.211679
GTACATG63350.033.0043071
TACATGG64750.030.4557652
ACATGGG67600.028.0977863
TGGTATC17750.027.77482
CATGGGG47200.025.4966754
GAGTACA49200.024.051781
TATACCG2552.7103233E-622.7440226
ATACCGC2252.6541325E-522.554497
AGTACAT52050.021.7292272
Produced by FastQC (version 0.11.5)