FastQCFastQC Report
Thu 2 Jun 2016
DRR028219_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028219_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2036241
Sequences flagged as poor quality0
Sequence length151
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC26150.046.846964
ATCAACG27050.045.5562553
GTATCAA30200.042.4847151
CAACGCA29350.041.986265
AACGCAG30350.041.0805366
GGTATCA18950.040.9299241
GTACATG61350.037.1006431
ACGCAGA34900.035.101667
TACATGG65350.033.7205282
GAGCGAC11000.032.94911145
TATCAAC37950.032.6625942
CGCAGAG36700.032.194968
ACATGGG67750.031.4560663
GTACGAC2405.2858013E-827.1830143
CATGGGG54300.025.364144
TCTCGGG20700.024.512863145
GCAGAGT48050.023.9866779
GAGTACA45750.022.9743521
GTACCGT1650.0026042721.9660746
AGTACAT48550.020.6042232
Produced by FastQC (version 0.11.5)