FastQCFastQC Report
Thu 2 Jun 2016
DRR028219_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028219_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2036241
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23760.11668559861038058No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23150.11368988248444069No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA29950.067.622161
GTATCAA49350.049.423531
TCAACGC54850.044.146534
ATCAACG54750.044.0947463
CAACGCA57600.042.0388455
AACGCAG60450.040.1767926
TATCAAC63800.037.8427162
ACGCAGA70800.033.8938987
GTGGTAT15700.033.2900541
CGCAGAG73300.032.5400858
GTACATG61400.031.4481351
TACATGG63750.028.5464612
GCAGAGT85100.027.8576819
ACATGGG65150.026.7069053
CAGAGTA82250.023.8869179
GAGTACA49600.023.8554481
CATGGGG50050.023.1762244
TGGTATC19800.021.6045682
TAGATCG1700.003094347221.3229775
AGTACAT52850.020.8524632
Produced by FastQC (version 0.11.5)