FastQCFastQC Report
Thu 2 Jun 2016
DRR028218_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028218_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1842001
Sequences flagged as poor quality0
Sequence length151
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGACC15350.056.67261145
TCAACGC22000.049.4275364
ATCAACG22750.047.7980583
CAACGCA25000.043.496235
GTATCAA26300.042.724431
GGTATCA16450.042.3063621
AACGCAG26350.040.7175336
GTACATG53000.039.392811
TACATGG55400.036.5085682
TGACGCG2052.928573E-1035.36279145
CCGACGC6900.034.67091145
TATCAAC32400.033.785652
ACATGGG57800.033.7384263
ACGCAGA30550.033.4586377
CGCAGAG31200.032.064538
GAGTACA39050.025.9900671
CATGGGG41850.025.1172964
GTATTAG14550.023.4172131
GCAGAGT41200.022.874239
GTACGGA2552.71118E-622.7431286
Produced by FastQC (version 0.11.5)