FastQCFastQC Report
Thu 2 Jun 2016
DRR028218_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028218_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1842001
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24380.13235606278172488No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24070.130673110383762No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA28400.066.225661
GTATCAA46250.052.5990521
TCAACGC51150.047.7649574
ATCAACG51800.047.0256353
CAACGCA53100.045.737825
AACGCAG55650.043.6420176
TATCAAC62000.039.4060942
ACGCAGA63700.037.557767
CGCAGAG65850.036.33158
GTACATG55350.033.9802861
TACATGG55600.032.206782
ACATGGG59850.030.0408863
GCAGAGT79700.029.4721539
GTGGTAT17700.027.8984781
TTTCGCG1658.424044E-526.3628589
GTCGTAC1701.02040474E-425.6299151
GAGTACA44150.024.178621
CATGGGG43600.023.9442464
TGGTATC20050.022.7798752
CCGGTCG3253.995774E-822.3070349
Produced by FastQC (version 0.11.5)