FastQCFastQC Report
Thu 2 Jun 2016
DRR028215_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028215_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1951677
Sequences flagged as poor quality0
Sequence length151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18400.047.6711431
GTATCAA28250.047.2157971
TCAACGC27700.042.9192434
ATCAACG27950.042.535353
GTACATG59900.039.2108421
CGACGAC10000.038.420578145
CAACGCA31650.037.7918555
TACATGG61250.037.3997732
AACGCAG33850.036.1922876
TATCAAC35450.034.3542942
ACATGGG68250.032.714183
ATCTACC16850.031.836096145
ACGCAGA37350.031.4421627
TGACGCG2351.2660166E-930.847511145
CGCAGAG39500.029.1801828
TCTCGGG20250.027.92271145
GAGTACA44850.026.1842751
CATGGGG52400.025.7317684
ACGATCA9400.024.678009145
AGTACAT46200.023.0655252
Produced by FastQC (version 0.11.5)