FastQCFastQC Report
Thu 2 Jun 2016
DRR028215_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028215_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1951677
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA28300.065.910361
GTATCAA45500.055.8294941
ATCAACG50000.049.877613
TCAACGC49950.049.6372644
CAACGCA53250.046.6972965
AACGCAG56800.044.033986
TATCAAC59250.042.216412
ACGCAGA65100.038.1970987
CGCAGAG67000.036.8974848
GTACATG64650.034.464891
TACATGG66550.031.9205322
ACATGGG67500.030.0726363
GTGGTAT15150.029.7020281
GCAGAGT82100.029.4048069
GAGTACA49850.025.3332631
CATGGGG50150.024.5750944
TATAACG1550.001808798523.3877742
TGGTATC17500.022.7863732
AGTACAT53500.022.2249432
CAGAGTA76350.019.6552479
Produced by FastQC (version 0.11.5)